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Tropism Analysis Package: Interactive Machine Learning Software to Identify Viral Host Factors Through Single-Cell Host-Virus mRNA Profiling
Authors: Kenny Pavan, Lamya Ben Ameur, Zach Goode, Emily Tiedemann, Elizabeth Kolb, Arpiar Saunders

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AAVdb

Interactive Single-Cell Viral Tropism Portal

Below are some example AAV tropism datasets generated using the Tropism Analysis Package (TAP). Please explore the datasets and feel free to reach out with any questions or suggestions!

Manuscript Methods
In-Vitro AAVs In-Vitro AAVs

A collection of 11 naturally occurring and engineered AAVs were used to transduce primary mixed mouse cortical cultures.

Single (1 rep; just neurons) Midbrain (4 reps) Cortex (3 reps; just neurons) Cortex (4 reps)
In-Vivo AAVs In-Vivo AAVs

14 barcoded AAVs introduced into the lateral ventricle of E13.5 mouse embryos. Samples were pooled and single-cell RNA-seq was performed.

Extended Use Case Extended Use Case SAD-B19ΔG

Rabies Virus SAD-B19ΔG was used to transduce primary mouse cortical cultures, then single-cell RNA-seq was performed and run through TAP.

Single Replicates